ATCC medium: 1753 von Hofsten & Malmqvist medium B
NaNO3 ........................2.0 g
K2HPO4 .......................0.5 g
MgSO4 . 7H2O .................0.2 g
CaCl2 . 2H2O ................20.0 mg
MnSO4 . H2O .................20.0 mg
FeSO4 . 7H2O ................20.0 mg
Glucose*.....................2.0 g
Distilled water..............1.0 L
Adjust medium for final pH 7.3. Autoclave at 121C for 15 minutes.
*For solid medium, substitute agar (15 g/L) for carbon source.