ATCC medium: 1907 Rhizomonas medium plus tetracycline
ATCC 1700 Rhizomonas medium
MgSO4 . 7H2O .......................0.5 g
KNO3 ...............................0.5 g
K2HPO4 .............................1.0 g
Glucose............................2.5 g
Trypticase Peptone (BD 211921).....5.0 g
Ca(NO3)2 . 4H2O....................60.0 mg
Agar, Noble (BD 214230)...........11.0 g
Distilled water....................1.0 L
Adjust medium to pH of 7.2. Autoclave at 121C for 15 minutes.
To above solution add
Tetracycline......................10.0 mcg
Filter-sterilize.