ATCC medium: 743 Alkvisco medium
Beef Extract (BD 212610)....10.0 g
Peptone.....................10.0 g
NaCl.........................5.0 g
KCN.........................10.0 mg
Acrylonitrile*...............0.5 g
Agar........................15.0 g
Distilled water..............1.0 L
Adjust pH to 6.5-8.0.
*Filter-sterilize the acrylonitrile solution and add to the sterile
basal medium which has been autoclaved at 121C for 10 minutes.
Acrylonitrile is a carcinogen; use appropriate precautions.