ATCC Medium: 2284 APT Medium w/ 0.3% Cysteine
Yeast Extract ………………………………………7.5 g
Tryptone……………………………………………12.5 g
Dextrose……………………………………………10.0 g
Sodium Citrate……………………………………..5.0 g
Thiamine HCl………………………………………100 mcg
K2HPO4……………………………………………5.0 g
MnCl2 ……………………………………………....0.14 g
MgSO4……………………………………………..0.8 g
FeSO4……………………………………………...0.04 g
Sorbitan Monooleate………………………………0.2 g
CystineHCl………………………………………….3.0 g
Agar (if required)…………………………………15.0 g
Distilled water……………………………………….1.0L
Autoclave at 121?C and dispense into appropriate vessel.