ATCC medium: 715 Trypticase-phytone-glucose medium with Tween 80 (2 ml/L)
Trypticase Peptone (BD 211921)....10.0 g
Phytone Peptone (BD 211906)........5.0 g
Glucose...........................15.0 g
Yeast extract......................2.5 g
Cysteine...........................0.5 g
K2HPO4 .............................2.0 g
MgCl2 ..............................0.5 g
ZnSO4* .............................0.25 g
FeCl3 ..............................2.0 mg
Tween 80...........................2.0 ml
Agar..............................15.0 g
Distilled deionized water..........1.0 L
*Dissolve separately in water and add to the bulk of the medium.
Autoclave medium 15 minutes at 121C.
Note: This is the formulation for ATCC Medium 549 with Tween 80 (2 ml/L) added.