ATCC Medium: 2721 Middlebrook 7H10 Agar w/ OADC Enrichment and 40mM Sodium Pyruvate
Base Medium
Middlebrook 7H10………………………………………19.0 g
Glycerol…………………………………………………..5.0 ml
Sodium Pyruvate………………………………………..4.4 g
DI Water………………………………………………….900.0 ml
Add 19 grams of dehydrated Middlebrook 7H10 medium and 4.4 grams sodium
pyruvate to 900 ml of water containing 5.0 ml of glycerol. Bring to a boil to
dissolve, and autoclave at 121?C. Cool to 50-55?C.
Aseptically add 100 ml of the following filter sterilized OADC Enrichment:
OADC Enrichment
Oleic Acid (Sigma Cat # O-1008)………………………..0.05 g
Albumin Fraction V Bovine (Sigma A9647-1KG)……...5 g
Dextrose (BD 215530)……………………….…………...2 g
Catalase (beef) (Sigma C-9322)………………………...0.004 g
NaCl………………………………………………………..0.85 g
DI Water……………………………………………………100 ml