ATCC medium: 1269 MMN (Marx 1969)
Malt extract.................3.0 g
D-Glucose...................10.0 g
(NH4)2C4H4O6 ..................0.25 g
KH2PO4 .......................0.5 g
MgSO4 . 7H2O .................0.15 g
CaCl2 ........................0.05 g
FeCl3 (1% solution) ..........1.2 ml
NaCl.........................0.025 g
Thiamine HCl...............100.0 mcg
Agar.........................1.5 %
Distilled water to...........1.0 L
Autoclave at 121C for 15 minutes.