ATCC medium: 1484 Citrate-phosphate-buffered glucose medium
Solution A:
(NH4)2SO4.....................3.0 g
KCl..........................0.1 g
MgSO4 . 7H2O .................0.5 g
Ca(NO3)2 . 4H2O...............0.02 g
FeSO4 . 7H2O ................10.0 mg
Citric acid (anhydrous)......1.92 g
Na2HPO4 ......................1.23 g
Distilled water............750.0 ml
Solution B:
Glucose.....................10.0 g
Yeast extract................1.0 g
Distilled water.............50.0 ml
Solution C:
Agarose (BRL)*...............6.0 g
Distilled water............200.0 ml
*Bethesda Research Laboratories, Ultra Pure electrophoresis grade is most suitable.
Autoclave Solutions A, B, and C separately. Cool to 55C.
Add Solutions B and C to Solution A, mix well and dispense immediately. The final pH will be 3.5.