ATCC medium: 1675 LB medium (ATCC medium 1065) with 10 mcg/ml chloramphenicol
ATCC Medium 1065 (see below) with 10.0 mcg/ml chloramphenicol
Prepare a filter-sterilized solution of chloramphenicol and aseptically add enough of it to the sterile basal medium to achieve the stated concentration.
ATCC Medium 1065: ATCC Medium 1065:
Tryptone (BD 211705)..............10.0 g
Yeast Extract (BD 212750)..........5.0 g
NaCl..............................10.0 g
Distilled water....................1.0 L
Autoclave at 121C for 15 minutes.