ATCC medium: 2123 Geothrix fermentans medium
NH4Cl ....................................0.25 g
NaH2PO4 ..................................0.60 g
Sodium acetate . 3H2O ....................1.36 g
NaHCO3 ...................................2.5 g
KCl......................................0.1 g
Wolfe's Vitamin Solution (see below)....10.0 ml
Wolfe's Mineral Solution (see below)....10.0 ml
NaNO3 ....................................0.90 g
Distilled water..........................1.0 L
Heat medium to a boil and allow to cool while sparging with 80% N2, 20% CO2. Adjust pH to 6.8-7.0.
Anaerobically dispense under same gas. Stopper and autoclave at 121C for 15 minutes.
Wolfe's Vitamin Solution:
Available from ATCC as a sterile ready-to-use liquid (Vitamin Supplement, catalog no. MD-VS).
Biotin.......................2.0 mg
Folic acid...................2.0 mg
Pyridoxine hydrochloride....10.0 mg
Thiamine . HCl...............5.0 mg
Riboflavin...................5.0 mg
Nicotinic acid...............5.0 mg
Calcium D-(+)-pantothenate...5.0 mg
Vitamin B12..................0.1 mg
p-Aminobenzoic acid..........5.0 mg
Thioctic acid................5.0 mg
Distilled water..............1.0 L