ATCC Medium: 1223 Medium for Treponema pectinovorum
Pectin...........................................................................0.8 g
K2HPO4 ......................................................................2.0 g
Polypeptone Peptone (BD 211910)……………….......5.0 g
Heart Infusion Broth (BD 238400)...............................5.0 g
Yeast Extract...............................................................5.0 g
NaCl............................................................................5.0 g
(NH4)2SO4.................................................................2.0 g
L-Cysteine . HCl..........................................................0.68 g
Agar, Bacto (BD 214050)............................................1.0 g
Resazurin (25 mg/100 ml water).................................4.0 ml
Rumen fluid................................................................500.0 ml
DI Water…………………………………………………500.0 ml
Adjust pH to 7.0 - 7.2 Pre-reduce and prepare medium anaerobically.
Inoculate under atmosphere of 90% N2, 10% CO2.