ATCC medium: 383 Haskins agar for Tetrahymena
Dextrin........................................8.0 g
Sodium acetate.................................0.6 g
Yeast extract..................................5.0 g
Liver Concentrate NF #XI Powder (ICN 100377)...0.6 g
Tryptone (BD 211705)...........................5.0 g
Agar..........................................16.0 g
Distilled water................................1.0 L
Adjust pH to 7.2 - 7.4. Dispense 2 ml amounts in 16 X 125 mm screwcapped test tubes.
Autoclave at 121C for fifteen minutes and slant in long slopes. After setting, aseptically dispense 2 ml sterile distilled water as overlay to cover slants completely.