ATCC Medium: 10 Nutrient Agar w/ Tap water and Soil Extract
Nutrient Agar (BD 213000)…………………………23 g
Tap Water……………………………………………750 ml
Soil Extract (See Below)…………………………….250 ml
Autoclave at 121oC. Soil
Extract African Violet Soil………………154.0 g
Na2CO3…………………………0.4 g
Tap Water……………………….400 ml
Boil for 1 hour. Filter to clarify before use. *Nutrient Agar is commercially available from BD, to make from scratch, follow formulation below:
BD Nutrient Agar Composition
Beef Extract……………………………..3.0 g
Peptone…………………………………5.0 g
Agar…………………………………….15.0 g
Final pH 6.8 +/- 0.2.