ATCC medium: 1416 4-hydroxybenzoic acid medium
Agar........................15.00 g
K2HPO4 . 3H2O.................4.25 g
NaH2PO4 . H2O.................1.00 g
NH4Cl ........................2.00 g
Nitrilotriacetic acid*.......0.10 g
MgSO4 . 7H2O .................0.20 g
FeSO4 . 7H2O ................12.0 mg
MnSO4 . H2O ..................3.0 mg
ZnSO4 . 7H2O .................3.0 mg
CoSO4 ........................1.0 mg
4-Hydroxybenzoic acid*.......1.00 g
Distilled water..............1.0 L
Adjust medium for final pH 7.2, +/- 0.2. Autoclave at 121C for 15 minutes.
*Dissolve in water made alkaline with concentrated NaOH, then readjust pH to 7.2 after addition of other ingredients.