ATCC medium: 412 Methanol medium for Achromobacter
Methanol*...................20.0 ml
NH4Cl ........................5.0 g
KH2PO4 .......................2.0 g
NaCl.........................0.5 g
MgSO4 ........................0.2 g
FeSO4 ........................2.0 mg
MnCl2 ........................2.0 mg
Yeast extract................0.2 g
Distilled water..............1.0 L
*Filter-sterilized and added aseptically to sterile medium Adjust pH to 7.0.
Autoclave at 121C for 15 minutes and then aseptically add methanol.