ATCC medium: 1282 Medium for sulfate reducers
Component I: MgSO4 ....................................2.0 g
Sodium citrate...........................5.0 g
CaSO4 ....................................1.0 g
NH4Cl ....................................1.0 g
Distilled water........................400.0 ml
Component II: K2HPO4 ...................................0.5 g
Distilled water........................200.0 ml
Component III: Sodium lactate...........................3.5 g
Yeast extract............................1.0 g
Distilled water........................400.0 ml
Adjust the pH of each component to 7.5 and autoclave at 121C for 15 minutes. Mix the three components aseptically and tube under 97% N2, 3% H2 while they are still warm to exclude as much oxygen as possible. Before inoculation, aseptically add the following filter-sterilized solutions:
5% Ferrous ammonium sulfate.............20.0 ml
Organic Acid Solution (see below).......10.0 ml
Wolfe's Vitamin Solution (see below)....10.0 ml
Wolfe's Mineral Solution (see below)....10.0 ml
Organic Acid Solution: Butyric acid.............................5.18 ml
Caproic acid.............................2.4 ml
Octanoic acid............................1.25 ml
Distilled water to......................50.0 ml
Adjust pH to 7.0 with 5 N NaOH and dilute to 100 ml with distilled water.