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ATCC medium: 1301 Marine peptone - succinate - salts medium

中國微生物菌種查詢網(wǎng) / 2017-10-11 11:34:09

ATCC medium: 1301 Marine peptone - succinate - salts medium (PSS) 
Peptone, Bacto (BD 211677)........ 10.0 g 
Succinic acid...................... 1.0 g 
(NH4)2SO4........................... 1.0 g 
MgSO4  . 7H2O ....................... 1.0 g 
FeCl3  . 6H2O ....................... 2.0 mg 
MnSO4  . H2O ........................ 2.0 mg 
Synthetic Seawater (see below)..... 1.0 L 
Synthetic Seawater:
NaCl ..............................27.5 g 
MgCl2 .............................. 5.0 g 
MgSO4  . 7H2O ....................... 2.0 g 
CaCl2 .............................. 0.5 g 
KCl................................ 1.0 g 
FeSO4 .............................. 1.0 mg 
Distilled water.................... 1.0 L 
Adjust pH to 6.8 with KOH. Autoclave at 121C for 15 minutes.

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