ATCC medium: 2261 Modified R2A medium
Yeast extract............................ 0.5 g
Proteose Peptone No. 3 (BD 211693)....... 0.5 g
Casamino acids........................... 0.5 g
Glucose.................................. 0.5 g
Tween 80 ................................ 0.1% (v/v)
Sodium pyruvate.......................... 0.3 g
K2HPO4 ................................... 0.3 g
MgSO4 . 7H2O ............................. 0.05 g
Agar.................................... 15.0 g
Distilled deionized water................ 1.0 L
Adjust pH to 7.2 with potassium phosphates before adding agar. Autoclave at 121C for 15 minutes.