ATCC medium: 1215 Acholeplasma medium
PPLO Broth w/o CV (BD 255420)................. 16.8 g
Distilled water.............................. 700.0 ml
Autoclave at 121C for 15 minutes. Cool to room temperature and add aseptically: Fetal bovine serum (heat-inactivated)........ 100.0 ml
Yeast Extract Solution (GIBCO 18180)......... 100.0 ml
0.1% Phenol Red............................... 20.0 ml
Tween-Glucose-BSA Solution (see below)....... 100.0 ml
Tween-Glucose-BSA Solution:
Glucose .......................................2.0 g
Tween 80....................................... 0.1 g
1% Bovine serum albumin fraction V........... 100.0 ml
Filter-sterilize solution through a 0.2 micrometer membrane filter.