ATCC Medium: 13 Azotobacter Supplement (Glucose/pH 6.0)
Base Medium
K2HPO4……………………………………1.0 g
MgSO4 x 7H20…………………………0.2 g
NaCl…………………………………………0.2 g
FeSO4…………………………………5.0 mg
Soil Extract (See Below)…………………100 ml
Glucose Solution (See Below)…………100 ml
Agar………………………………………15.0 g
Tap Water……………………………800 ml
Adjust base medium to pH 6.0 Autoclave at 121oC.
Glucose Solution
Glucose………………………20 g
DI Water……………………100 ml
Filter sterilize and add to sterile base.
Soil Extract
African Violet Soil……………77.0 g
Na2CO3………………………0.2 g
DI water………………………200 ml
Boil for 1 hour. Filter to clarify before use.