ATCC medium: 1558 Desulfurococcus medium
Solution A:
(NH4)2SO4..................... 1.3 g
KH2PO4 ....................... 0.28 g
MgSO4 . 7H2O ................. 0.25 g
CaCl2 . 2H2O ................. 0.07 g
FeSO4 . 7H2O ................ 28.0 mg
MnCl2 . 4H2O ................. 1.8 mg
Na2B4O7 . 10H2O ............... 4.5 mg
ZnSO4 . 7H2O ............... 220.0 mcg
CuCl2 .2H2O ................. 50.0 mcg
Na2MoO4 . 2H2O............... 30.0 mcg
VOSO4 . 2H2O ................ 30.0 mcg
CoSO4 . 7H2O ................ 10.0 mcg
Distilled water............ 300.0 ml
Autoclave at 121C for 15 minutes. Equilibrate under nitrogen gas.
Solution B:
Sulphur...................... 5.0 g
Distilled water............ 450.0 ml
Autoclave at 100C for 30 minutes for 3 consecutive days. Equilibrate under nitrogen gas.
Solution C:
Tryptone (BD 211705)......... 2.0 g
Yeast extract................ 2.0 g
Resazurin.................... 1.0 mg
Distilled water............ 500.0 ml
Autoclave at 121C for 15 minutes. Equilibrate under nitrogen gas.
Solution D:
Na2S . 9H2O.................. 0.5 g
Distilled water............. 50.0 ml
Autoclave at 121C for 15 minutes under nitrogen gas. Aseptically combine Solutions A through D under nitrogen gas. Seal containers with butyl rubber stoppers.