ATCC medium: 1562 Standard agar with methanol and yeast extract
Yeast extract...................... 0.5 g
Agar (if necessary)............... 20.0 g
Distilled water.................. 982.0 ml
Autoclave at 121C for 15 minutes. Cool to 50C and add aseptically the following sterilized solutions:
Solution A (see below)............. 1.0 ml
Solution B (see below)............. 5.0 ml
Solution C (see below)............. 1.0 ml
Solution D (see below)............. 1.0 ml
Methanol, filter-sterilized....... 10.0 ml
Adjust pH aseptically, if necessary, to 4.0-4.5.
SolutionA:
KH2PO4............................68.05 g
K2HPO4 ............................ 87.09 g
Distilled water.................... 1.0 L
Solution.B:
NH4Cl........................... 152.28 g
Distilled water.................... 1.0 L
Solution C:
CaCl2 . 6H2O ....................... 5.47 g
Distilled water.................... 1.0 L
Solution D:
MgSO4 . 7H2O g ...................... 71.2 g
ZnSO4 . 7H2O ....................... 0.44
MnSO4 . 4H2O ....................... 0.812 g
CuSO4 . 5H2O ....................... 0.785 g
Na2MoO4 . 2H2O...................... 0.252 g
FeSO4 . 7H2O ....................... 4.98 g
Distilled water to................. 1.0 L