ATCC medium: 305 Coprinus medium
Glucose........................... 20.0 g
Asparagine......................... 2.0 g
Salt Solution (see below)......... 25.0 ml
Thiamine Solution (see below)...... 1.0 ml
Yeast extract...................... 0.75 g
Casein hydrolysate................. 0.75 g
Malt extract....................... 0.60 g
Agar.............................. 20.0 g
Distilled water.................... 1.0 L
Autoclave at 121C for 15 minutes. After autoclaving, pH is 6.8.
Salt Solution:
Ammonium tartrate ................. 10.0 g
KH2PO4 ............................ 20.0 g
Na2HPO4 ........................... 45.0 g
Na2SO4 . 10H2O...................... 5.6 g
Distilled water.................. 500.0 ml
Thiamine..........................Solution: 10.0 mg
Thiamine Distilled water.................. 100.0 ml
Filter-sterilize and add aseptically.