ATCC medium: 250 Trypticase soy agar with 3% NaCl, 10% inactivated horse serum and 1000 U/ml penicillin
Prepare Trypticase Soy Agar (BD 211043) per manufacturer’s instructions. Add 30 g of NaCl to BD 211043. Aseptically add horse serum and filter-sterilized penicillin to sterile basal medium.