ATCC medium: 920 Chloroflexus medium
Nitrilotriacetic acid.................... 0.1 g
Micronutrient Solution (see below)....... 1.0 ml
FeCl3 Solution (see below) ............... 1.0 ml
CaSO4 . 2H2O ............................. 0.06 g
MgSO4 . 7H2O ............................. 0.10 g g
NaCl..................................... 0.008
KNO3 ..................................... 0.103 g
NaNO3 .................................... 0.689 g
Na2HPO4 .................................. 0.111 g
NH4Cl .................................... 0.2 g
Yeast extract............................ 0.5 g
Glycyl-glycine........................... 0.5 g
Distilled water.......................... 1.0 L
Prepare above medium and adjust pH to 8.2-8.4. Add 0.5 g sodium sulfide. Readjust pH to 8.2-8.4. For broth, filter-sterilize and dispense in tubes. For solid medium add 15 grams agar.
FeCl3 Solution
FeCl3 ..............................: 0.2905 g
Distilled water.................... 1.0 L
Micronutrient Solution:.
H2SO4 (concentrated).............. 0.5 ml
MnSO4 . 7H2O ....................... 2.28 g
ZnSO4 . 7H2O ....................... 0.50 g
H3BO3 .............................. 0.50 g
CuSO4 . 2H2O ....................... 0.025 g
Na2MoO4 . 2H2O...................... 0.025 g
CoCl2 . 6H2O ....................... 0.045 g
Distilled water.................... 1.0 L