ATCC medium: 225 Beef extract medium
Beef extract................ 10.0 g
Peptone..................... 10.0 g
NaCl......................... 5.0 g
Agar (if needed)............ 25.0 g
Distilled water.............. 1.0 L
Adjust pH to 7.2.
Autoclave at 121C for 15 minutes.