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ATCC Medium: 43 Clostridium Medium

中國(guó)微生物菌種查詢(xún)網(wǎng) / 2017-08-21 12:38:00

ATCC Medium: 43 Clostridium Medium 

K2HPO4…………………………………..1.75 g 

CaCl2 x 2H2O…………………………….0.01 g 

MgSO4 x 7H2O…………………………...0.2 g 

FeSO4 x 7H2O……………………………0.01 g 

L-ArginineHCl………………….…………2.0 g 

L-LysineHCl………………………………2.0 g 

Yeast Extract……………………………...5.0 g 

Sodium Formate………..….……………..2.0 g 

NH4Cl………………………………………2.0 g 

Methylene Blue (1mg/ml solution)….…..2.0 ml 

Na2S x 9H2O Solution (see below)…….50 ml 

Agar…………………………………..……15 g 

Tap water……………………….…………950 ml 

Autoclave at 121oC. Na2S x 9H2O Solution 

Na2S x 9H2O………………………………0.3 g 

DI Water……………………………………50 ml 

Dispense anaerobically under 100% N2.

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