ATCC Medium: 9 Nutrient Agar w/ 2% Yeast Extract
Nutrient Agar (BD 213000)…………………23.0 g
Yeast Extract………………………………...20.0 g
DI Water…………………………………….1000 ml
Autoclave at 121oC.
*Nutrient Agar is commercially available from BD, to make from scratch, follow formulation below:
BD Nutrient Agar Composition
Beef Extract……………………………..3.0 g
Peptone…………………………………5.0 g
Agar…………………………………….15.0 g
Final pH 6.8 +/- 0.2.