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ATCC medium: 2382 Hagerdahl medium

中國微生物菌種查詢網(wǎng) / 2017-06-28 08:39:51

ATCC medium: 2382 Hagerdahl medium 

Macro Salts 10X (per liter): 

NaCl........................15.0 g 

(NH4)2SO4....................31.0 g 

Na2HPO4* ....................91.0 g 

KH2PO4 .......................9.0 g 

*Na2HPO4 . 7H2O, 172.0 g, may be used. 

Adjust pH to 7.4 +/- 0.2. 

Microsalts 50X (per 500 ml): EDTA.........................1.25 g 

MgSO4 . 7H2O..................5.0 g 

ZnSO4 . 7H2O..................0.2 g

FeSO4 . 7H2O..................0.5 g 

MnSO4 . H2O...................0.38 g 

CaCl2 . 2H2O..................0.66 g 

Carbon Source (choose one): 

Cellobiose*..................5.0 g/L 

Glucose......................5.0 g/L 

Xylan........................7.5-10.0 g/L 

*(The organism prefers cellobiose.) Additions: 

Biotin.......................1.0 mg/L 

Thiamine ....................1.0 mg/L 

Yeast extract**..............0.2 g/L 

**(optional-not at all necessary) 

Autoclave 10X Macro Salts and solid carbon source together. Cool and add sterile Microsalts to 1X, Soluble carbon source and “Additions”.

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