ATCC medium: 1409 Modified Imhoff's medium
NaCl..............................30.0 g
SLA Trace Elements (see below).....1.0 ml
VA Vitamins (see below)............1.0 ml
KH2PO4 .............................1.0 g
MgCl2 . 6H2O .......................0.5 g
CaCl2 . 2H2O .......................0.1 g
NH4Cl ..............................1.0 g
NaHCO3 .............................3.0 g
Na2SO4 .............................0.7 g
Sodium acetate.....................1.0 g
Sodium ascorbate...................0.5 g
Yeast extract......................0.1 g
Sodium sulfide*..................156.0 mg
Distilled water....................1.0 L
Adjust medium for final pH 6.9-7.0. *Sterilize sodium sulfide separately and add aseptically to sterile medium.
SLA Trace Elements:
CoCl2 . 6H2O .....................250.0 mg
NiCl2 . 6H2O ......................10.0 mg
CuCl2 . 2H2O ......................10.0 mg
MnCl2 . 4H2O ......................70.0 mg
ZnCl2 ............................100.0 mg
H3BO3 ............................500.0 mg
Na2MoO4 . 2H2O.....................30.0 mg
Na2SeO3 . 5H2O.....................10.0 mg
FeCl2 . 4H2O .......................1.8 g
Distilled water....................1.0 L
Adjust trace element solution to pH 2-3.
VA Vitamins: Biotin............................50.0 mg
Nicotinamide.....................175.0 mg
Thiamine . HCl...................150.0 mg
p-Aminobenzoic acid..............100.0 mg
Pyridoxine . HCl..................50.0 mg
Calcium D-(+)-pantothenate........50.0 mg
Cyanocobalamin....................25.0 mg
Distilled water..................500.0 ml