ATCC Medium: 2737 Leeming & Notman agar Modified MLNA
media:
Bacteriological peptone……………………….. 10g
Glucose………………………………………... 10g
Yeast extract…………………………………… 2.0 g
Ox bile, desiccated……………………………. 8.0g
Glycerol……………………………………… 10mL
Glycerol monostearate………………………….. 0.5g
Tween 60……………………………………….. 5.0mL
Olive oil………………………………………… 20mL
Agar…………………………………………….. 15g
Deionized water………………………………… 1.0 L
Medium is sterilized by autoclaving at 110C for 20 minutes. For liquid media, omit the agar.