1368 Clostridium Cellulolytic medium
K2HPO4 x 3H2O …………………………...2.9 g
KH2PO4…………………………………….1.5 g
(NH4)2SO4…………………………………1.3 g
MgCl2 x 6H2O……………………………...1.0 g
CaCl2 x 2H2O………………………………0.15 g
FeSO4……………………………………….1.25 mg or ( 1.25ml of mg/ml solution)
Yeast Extract………………………………..2.0 g
Cellulose…………………………………….7.5 g
Resazurin……………………………………2.0 ml
Cysteine HCl (add after boiling)……………1.0 g
DI Water…………………………………….1.0 L
Adjust final pH to 7.2. Prepare medium, dispense under a constant flow of nitrogen, stopper and autoclave 15 minutes. For preparation of solid media add 20g of agar, adjust pH to 7.5 with NaOH.