ATCC Medium: 1021 SCGYEM Medium
Isoelectric Casein (Difco 0145)……………………10.0 g
Glucose………………………………………………..2.5 g
Yeast Extract………………………………………….5.0 g
Na2HPO4……………………………………………1.325 g
KH2PO4………………………………………………..0.8 g
DI Water…………………………………………….900.0 ml
Heat inactivated fetal bovine serum……………..100.0 ml
The components, with the exclusion of serum, are combined and autoclaved at 15 lbs., 121C for 30 minutes.
The serum is added aseptically after the solution has cooled. Dispense 3.0 ml per 16 x 125 mm screw-cap tubes.