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ATCC medium: 2106 Dechlorimonas agitata medium

中國微生物菌種查詢網 / 2017-01-11 09:17:47

ATCC medium: 2106 Dechlorimonas agitata medium

NH4Cl ....................................0.25 g

NaClO3 ...................................1.03 g

NaH2PO4 ..................................0.60 g

Sodium acetate . 2H2O ....................1.36 g

NaHCO3 ...................................2.5 g

KCl......................................0.1 g

Wolfe's Vitamin Solution (see below)....10.0 ml

Wolfe's Mineral Solution (see below)....10.0 ml

Distilled water........................980.0 ml

Combine ingredients (except for Wolfe's Vitamin Solution). Boil, then cool under flowing 80% N2, 20% CO2.

Aseptically add 10.0 ml of vitamin solution. After gassing for at least 15 minutes, adjust pH to between 7.0-7.5.

Dispense into aluminum seal tubes under gas. Wolfe's Mineral Solution: Available from ATCC as a sterile ready-to-use liquid (Trace Mineral Supplement, catalog no. MD-TMS.)

Nitrilotriacetic acid........1.5 g

MgSO4 . 7H2O .................3.0 g

MnSO4 . H2O ..................0.5 g

NaCl.........................1.0 g

FeSO4 . 7H2O .................0.1 g

CoCl2 . 6H2O .................0.1 g

CaCl2 ........................0.1 g

ZnSO4 . 7H2O .................0.1 g

CuSO4 . 5H2O .................0.01 g

AlK(SO4)2 . 12H2O.............0.01 g

H3BO3 ........................0.01 g

Na2MoO4 . 2H2O................0.01 g

Distilled water..............1.0 L

Add nitrilotriacetic acid to approximately 500 ml of water and adjust to pH 6.5 with KOH to dissolve the compound. Bring volume to 1.0 L with remaining water and add remaining compounds one at a time. 2

Wolfe's Vitamin Solution:

Available from ATCC as a sterile ready-to-use liquid (Vitamin Supplement, catalog no. MD-VS).

Biotin.......................2.0 mg Folic acid...................2.0 mg

Pyridoxine hydrochloride....10.0 mg

Thiamine . HCl...............5.0 mg

Riboflavin...................5.0 mg

Nicotinic acid...............5.0 mg

Calcium D-(+)-pantothenate...5.0 mg

Vitamin B12..................0.1 mg

p-Aminobenzoic acid..........5.0 mg

Thioctic acid................5.0 mg

Distilled water..............1.0 L

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