ATCC medium: 1288 N plus C medium
Citric acid . H2O ..................4.04 g
FeCl2 . 4H2O .......................0.06 g
MgSO4 . 7H2O .......................0.6 g
CaCl2 . 2H2O .......................0.6 g
ZnSO4 . 7H2O .......................0.034 g
Tryptone (BD 211705)..............10.0 g
Dextrose..........................10.0 g
Yeast Extract (BD 212750)..........1.5 g
KH2PO4 .............................2.0 g
Distilled water....................1.0 L
Dissolve components one at a time in distilled water. Adjust to pH 4.6 with KOH.
Autoclave at 121C for 15 minutes. Prior to inoculation add 0.1 ml Hemin solution per 100 ml of medium.
Hemin Solution: Hemin............................250.0 mg
NaOH...............................1.0 g
Distilled water..................100.0 ml
Autoclave 15 minutes at 121C.