ATCC Medium: 958 Hemin Medium for Mycobacterium
Middlebrook 7H10………………………………………….….19.0 g
Glycerol…………………………………………………….…...5.0 ml
Dubos Oleic Albumin Complex (OADC) (see below)……...100.0 ml
DI Water………………………………………………….……..900.0 ml
Add 60 micro moles of Hemin* and mix all components except for OADC. Bring to a boil to dissolve agar and autoclave at 121?C.
Cool to 50-55?C and aseptically OADC. *Hemin must be solubilized with 1 N NaOH.
To make broth medium, utilize Middlebrook 7H9 formulation (MD-1395) and add 60 micro moles of Hemin.
OADC Oleic Acid (Sigma Cat # O-1008)……………………………..0.05 g
Albumin Fraction V Bovine (Sigma A9647)……….………...5 g
Dextrose (BD 215530)……………………………………....…2 g
Catalase (beef) (Sigma C-9322)……………………………...0.004 g
NaCl……………………………………………………………..0.85 g
DI Water…………………………………………………………100 ml
OADC is available from BD (237510) or can be made following the formulation above. Final Product Description
? Medium should be translucent and brown/slightly dark green in color.
? Recommended storage temperature: 2-8?C.