Human Coxsackievirus B3 ATCC病毒類(lèi)
小楊 / 2019-11-07 09:16:08
基本信息
平臺(tái)編號(hào)：bio-106263
規(guī)格：frozen
拉丁屬名：Human Coxsackievirus B3
中文譯名：人柯薩奇B3病毒
詳情介紹
Human Coxsackievirus B3 拉丁名
ATCC VR-30 編號(hào)
Classification Picornaviridae, Enterovirus, Human enterovirus B
Deposited As Coxsackievirus B3
Agent Human Coxsackievirus B3
Strain Nancy
Biosafety Level 安全等級(jí) 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Cryopreserved in 10% FBS.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
LLC-MK2 (ATCC CCL-7.1
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度 : 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days
Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor JL Melnick
Source Stool from febrile patient with minor illness, Connecticut, 1949
References 參考文獻(xiàn)
Melnick JL, Ledinko N. Immunological reactions of the Coxsackie viruses. I. The neutralization test; technic and application. J. Exp. Med. 92: 463-482, 1950. PubMed: 14778925
Cross References
Nucleotide (GenBank) : AJ295194 Human coxsackievirus B3 genomic RNA for partial polyprotein gene, isolate VR-30, ATCC.
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