北京百歐博偉生物談?wù)?亞硝化單胞菌 ATCC BAA-120的培養(yǎng)注意事項(xiàng)
Nitrosomonas europaea Winogradsky 菌株拉丁名
(ATCC? BAA-1201?) 菌株編號(hào)
Strain Designations菌株別名: ML1 /
Type Strain 模式菌株: no /
Biosafety Level 生物安全等級(jí) : 1
Strain Designations 菌株別名 ML1
Isolation 分離源 Sludge Wisconsin, United States isolation date: September, 2003
Type Strain 模式菌株no
Biosafety Level 生物安全等級(jí) 1
Product Format 提供形式 frozen
Medium 培養(yǎng)基 ATCC? Medium 2265: Nitrosomonas europaea medium
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度 : 30.0℃
Name of Depositor 寄存人 DR Noguera, H Park Special Collection NSF - Bacteriology
Isolation Sludge Wisconsin, United States
isolation date: September, 2003
Year of Origin 收藏年份 2003
References 參考文獻(xiàn) Park HD, Noguera DR. Evaluating the effect of dissolved oxygen on ammonia-oxidizing bacterial communities in activated sludge.. Water Res. 38: 3275-3286, 2004. PubMed: 15276744
chemostat reactor seeded with activated sludge, Marshall wastewater treatment plant, Marshall, WI
其中開通海外直郵業(yè)務(wù)專業(yè)代理美國ATCC微生物菌種保藏中心菌種細(xì)菌和噬菌體（18380）細(xì)胞系和雜交瘤（5004）；真菌和酵母（58208）
原代細(xì)胞（143）；原生動(dòng)物和藻類（2426）
干細(xì)胞（102）；載體，克隆與分子（18005）；病毒（3598）除了專利和受控產(chǎn)品其他均可供應(yīng)，全方位滿足您實(shí)驗(yàn)需求，另到貨時(shí)間短COA齊全。
Medium 培養(yǎng)基 ATCC? Medium 18: Trypticase Soy Agar/Broth
那么培養(yǎng)時(shí)間應(yīng)該注意什么呢？
Propagation Procedure
1.Open vial according to enclosed instructions.
2.From a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3.Use 0.1 ml of this suspension to inoculate #18 slants and 0.1ml to inoculate #18 plates.
4.Incubate tubes and plates at 30oC, under aerobic conditions, for 24 hours.
5.After 24 hours of incubation, wash cells from the slant and transfer this broth to a new slant and plate. Incubate another 24 hours under aerobic conditions. This second transfer and incubation is necessary for complete removal of the cryoprotectant, which can inhibit growth.
1.根據(jù)隨附的說明打開樣品瓶。
2.從＃18肉湯（5至6毫升）的單管中，用巴斯德或1.0毫升移液管取出約0.5至1.0毫升，并用于再水化整個(gè)顆粒。
3.使用0.1ml該懸浮液接種＃18斜面和0.1ml接種＃18板。
4.在有氧條件下，在30℃下孵育管和板24小時(shí)。
5.孵育24小時(shí)后，從斜面洗滌細(xì)胞并將該肉湯轉(zhuǎn)移到新的斜面和平板上。 在有氧條件下再孵育24小時(shí)。 這種第二次轉(zhuǎn)移和溫育對(duì)于完全除去可以抑制生長(zhǎng)的冷凍保護(hù)劑是必需的。