原核抗性 | Amp |
克隆菌株 | DH5a |
培養(yǎng)條件 | 37度 |
載體宿主 | 枯草桿菌 |
載體用途 | 蛋白表達(dá) |
基因類型 | ORF |
原核抗性 | Amp |
質(zhì)粒簡(jiǎn)介 | pSTOP1622質(zhì)粒是一個(gè)枯草桿菌載體,Bacillus megaterium is a rod-shaped, non-pathogenic Gram-positive bacterium, which is able to grow on a wide variety of carbon sources. Like other soil bacteria, it is able to secrete high amount of protein into the culture medium (up to 0.5 g/L of recombinant protein). This property, together with its ability to grow in many ecological niches such as waste from meat industry, melasse or petrochemical effluents makes it an interesting organism for industrial-scale production of recombinant proteins. In addition to B. subtilis, it has been used as Gram-positive model organism for studying several research topics such as biochemistry, sporulation, and bacteriophages. |