| 啟動子 | CMV |
| 復(fù)制子 | pUC |
| 質(zhì)粒大小 | 7567bp |
| 原核抗性 | Amp |
| 篩選標(biāo)記 | EGFP |
| 克隆菌株 | Stbl3 |
| 培養(yǎng)條件 | 37度 |
| 載體宿主 | 哺乳細(xì)胞,慢病毒 |
| 載體用途 | 干擾沉默 |
| 基因類型 | shRNA |
| 原核抗性 | Amp |
| 熒光蛋白 | 綠色 |
| 質(zhì)粒簡介 |
EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
pSico shRNA cloning protocol |