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平臺(tái)編號(hào)：bio-82357
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質(zhì)粒信息： 宿主：DH5α，BL21
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規(guī)格：2ml甘油管 20ul質(zhì)粒
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用途：/
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
pIEFBPR(同源臂有堿基突變，不影響功能）
基本信息
復(fù)制子: ColE1 Ori
質(zhì)粒分類(lèi): 枯草桿菌質(zhì)粒；枯草整合質(zhì)粒；枯草分泌質(zhì)粒
質(zhì)粒大小: 8965bp
原核抗性: Amp
克隆菌株: DH5a
培養(yǎng)條件: 37度
質(zhì)粒屬性
載體宿主： 枯草桿菌
載體用途： 蛋白表達(dá)
基因種屬：
基因類(lèi)型： ORF
原核抗性： Amp
真核抗性：
熒光蛋白：
質(zhì)粒簡(jiǎn)介
pIEFBPR質(zhì)粒是一個(gè)枯草整合型分泌載體，該質(zhì)粒的參考文獻(xiàn)：mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis
ColE1 ori, ColE1 replication origin; amp, ampicillin-resistance marker; spc, spectinomycin-resistance marker; lacI, lac-repressor-encoding gene; Pspac, Pspac promoter; mazF, E.coli toxin gene; Plpp, promoter of E.coli lpp gene; mazE, E.coli antitoxin gene; DR, directly-repeated sequence; amyE-front and amyE-back, upstream and downstream sequences of the B.subtilis amyE gene, respectively; bpr-front and bpr-back, upstream and downstream sequences of the B.subtilis bpr gene, respectively. All of the DNA sequences located between the amyE-front and amyE-back or the bpr-front and bpr-back allow integration into the chromosome of B.subtilis via a double-crossover event, resulting in selection for spectinomycin-resistance. The unique restriction enzyme sites are highlighted in blue.
復(fù)制子: ColE1 Ori
質(zhì)粒分類(lèi): 枯草桿菌質(zhì)粒；枯草整合質(zhì)粒；枯草分泌質(zhì)粒
質(zhì)粒大小: 8965bp
原核抗性: Amp
克隆菌株: DH5a
培養(yǎng)條件: 37度
質(zhì)粒屬性
載體宿主： 枯草桿菌
載體用途： 蛋白表達(dá)
基因種屬：
基因類(lèi)型： ORF
原核抗性： Amp
真核抗性：
熒光蛋白：
質(zhì)粒簡(jiǎn)介
pIEFBPR質(zhì)粒是一個(gè)枯草整合型分泌載體，該質(zhì)粒的參考文獻(xiàn)：mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis
ColE1 ori, ColE1 replication origin; amp, ampicillin-resistance marker; spc, spectinomycin-resistance marker; lacI, lac-repressor-encoding gene; Pspac, Pspac promoter; mazF, E.coli toxin gene; Plpp, promoter of E.coli lpp gene; mazE, E.coli antitoxin gene; DR, directly-repeated sequence; amyE-front and amyE-back, upstream and downstream sequences of the B.subtilis amyE gene, respectively; bpr-front and bpr-back, upstream and downstream sequences of the B.subtilis bpr gene, respectively. All of the DNA sequences located between the amyE-front and amyE-back or the bpr-front and bpr-back allow integration into the chromosome of B.subtilis via a double-crossover event, resulting in selection for spectinomycin-resistance. The unique restriction enzyme sites are highlighted in blue.
