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平臺編號：bio-82357
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質(zhì)粒信息： 宿主：DH5α，BL21
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規(guī)格：2ml甘油管 20ul質(zhì)粒
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用途：/
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
pIEFBPR(同源臂有堿基突變，不影響功能）
| 復(fù)制子 | ColE1 Ori |
| 質(zhì)粒分類 | 枯草桿菌質(zhì)粒；枯草整合質(zhì)粒；枯草分泌質(zhì)粒 |
| 質(zhì)粒大小 | 8965bp |
| 原核抗性 | Amp |
| 克隆菌株 | DH5a |
| 培養(yǎng)條件 | 37度 |
| 載體宿主 | 枯草桿菌 |
| 載體用途 | 蛋白表達 |
| 基因類型 | ORF |
| 原核抗性 | Amp |
| 質(zhì)粒簡介 | pIEFBPR質(zhì)粒是一個枯草整合型分泌載體，該質(zhì)粒的參考文獻：mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis ColE1 ori, ColE1 replication origin; amp, ampicillin-resistance marker; spc, spectinomycin-resistance marker; lacI, lac-repressor-encoding gene; Pspac, Pspac promoter; mazF, E.coli toxin gene; Plpp, promoter of E.coli lpp gene; mazE, E.coli antitoxin gene; DR, directly-repeated sequence; amyE-front and amyE-back, upstream and downstream sequences of the B.subtilis amyE gene, respectively; bpr-front and bpr-back, upstream and downstream sequences of the B.subtilis bpr gene, respectively. All of the DNA sequences located between the amyE-front and amyE-back or the bpr-front and bpr-back allow integration into the chromosome of B.subtilis via a double-crossover event, resulting in selection for spectinomycin-resistance. The unique restriction enzyme sites are highlighted in blue. |
