>中國微生物菌種>國內(nèi)微生物菌種資源>珊瑚球菌
|
菌種名稱 |
珊瑚球菌 |
| 拉丁文名 | Corallococcus sp. |
|
其他編號 |
8#-3 |
| 培養(yǎng)基編號 | 169 |
| 培養(yǎng)溫度 | 30℃ |
| 名稱 | VY/2 AGAR |
| 備注 | Baker's yeast 5.00 g Vitamin B12 0.50 mg CaCl2 x 2 H2O 1.36 g Agar (Difco) 15.00 g Distilled water 1000.00 ml Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50℃ (use pH-indicator paper). For suspension of freeze-dried cells from ampoules add about 0.5 - 1.0 ml medium MD1 (per liter: casiton 3.0 g; calciumchloride dihydrate 0.7 g; magnesiumsulphate heptahydrate 2.0 g) to the vial with freeze dried material. |