| 啟動子 | CMV |
| 復(fù)制子 | pUC ori |
| 終止子 | β-globin poly(A) |
| 質(zhì)粒大小 | 8890bp |
| 原核抗性 | Amp |
| 克隆菌株 | Stbl3 |
| 培養(yǎng)條件 | 37℃，有氧 LB |
| 表達宿主 | 哺乳細胞 |
| 誘導(dǎo)方式 | 無須誘導(dǎo)，瞬時表達 |
| 5'測序引物 | CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 3'測序引物 | 根據(jù)序列設(shè)計引物 |
| 質(zhì)粒簡介 | This 3rd generation LV packaging plasmid includes gag, coding for the virion main structural proteins; pol, responsible for the retrovirus-specific enzymes; and RRE, a binding site for the Rev protein which facilitates export of the RNA from the nucleus. This plasmid does not include Rev. The third generation packaging system offers maximal biosafety but is more cumbersome, as it involves the transfection of four different plasmids in the producer cells. |
