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平臺(tái)編號(hào)：bio-134064
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質(zhì)粒信息： pMDLg-pRRE
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規(guī)格：2ml甘油管 20ul質(zhì)粒
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用途：//
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu) 說(shuō)明書(shū)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
pMDLg-pRRE
基本信息
啟動(dòng)子:CMV
復(fù)制子:pUC ori
終止子:β-globin poly(A)
質(zhì)粒大小:8890bp
原核抗性:Amp
克隆菌株:Stbl3
培養(yǎng)條件:37℃，有氧 LB
表達(dá)宿主:哺乳細(xì)胞
誘導(dǎo)方式:無(wú)須誘導(dǎo)，瞬時(shí)表達(dá)
5'測(cè)序引物:CMV-F：CGCAAATGGGCGGTAGGCGTG
3'測(cè)序引物:根據(jù)序列設(shè)計(jì)引物
質(zhì)粒簡(jiǎn)介
This 3rd generation LV packaging plasmid includes gag, coding for the virion main structural proteins; pol, responsible for the retrovirus-specific enzymes; and RRE, a binding site for the Rev protein which facilitates export of the RNA from the nucleus. This plasmid does not include Rev. The third generation packaging system offers maximal biosafety but is more cumbersome, as it involves the transfection of four different plasmids in the producer cells.
