| 別稱 | Plasmid#13573;金黃葡萄球拷貝整合 |
| 復(fù)制子 | Ec |
| 質(zhì)粒大小 | 5505bp |
| 原核抗性 | Amp |
| 篩選標(biāo)記 | Chl |
| 克隆菌株 | DH5a |
| 培養(yǎng)條件 | 37度 |
| 表達(dá)宿主 | 金黃色葡萄球菌 |
| 5'測(cè)序引物 | 根據(jù)全序列設(shè)計(jì) |
| 3'測(cè)序引物 | 根據(jù)全序列設(shè)計(jì) |
| 載體宿主 | 金黃色葡萄球菌 |
| 原核抗性 | Amp |
| 質(zhì)粒簡(jiǎn)介 | pLI50質(zhì)粒是一個(gè)金黃色葡萄球菌單拷貝整合載體，在大腸桿菌里氨芐抗性，在金黃色葡萄球菌里是氯霉素抗性。參考文獻(xiàn)：Construction of single-copy integration vectors for Staphylococcus aureus.
Single-copy integration vectors suitable for cloning in Staphylococcus aureus have been constructed. Their construction was based on the site-specific recombination system of staphylococcal phage, L54a. The vectors are capable of autonomous replication in Escherichia coli, but they are not endowed with a replication function in S. aureus. As a consequence, establishment of these vectors in S. aureus can only be achieved by the integration system of the phage. Once integrated into the chromosome, the vectors, or their derivatives, were stably inherited even without selective pressure. Because such a vector exists in an integrated form in S. aureus, the gene dosage of the DNA cloned in the vector matches that of the chromosome. |
