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 基本信息
復(fù)制子:pUC ori,f1 ori終止子:SV40 poly(A) signal質(zhì)粒分類:哺乳細胞，信號通路報告載體質(zhì)粒大小:4818bp原核抗性:Amp克隆菌株:DH5α培養(yǎng)條件:37℃，有氧 LB表達宿主:哺乳細胞誘導(dǎo)方式:無須誘導(dǎo)，瞬時表達5'測序引物:RVP3:CTAGCAAAATAGGCTGTCCC3'測序引物:根據(jù)序列設(shè)計引物
質(zhì)粒簡介    The pGL3 Luciferase Reporter Vectors(a–c) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, these Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.
質(zhì)粒圖譜 

質(zhì)粒序列

LOCUS       Exported File           4818 bp ds-DNA    circular SYN 23-3-2015

DEFINITION  .

ACCESSION   .

VERSION     .

KEYWORDS    pGL3-basic

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4818)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported 2015-3-23 from MLCC

FEATURES             Location/Qualifiers

     source          1..4818

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             88..1740

                     /codon_start=1

                     /gene="luc+"

                     /product="firefly luciferase"

                     /EC_number="

                     "

                     /note="luciferase"

                     /note="enhanced?luc+ version of the luciferase gene"

                     /protein_id="

                     "

                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA

                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP

                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS

                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA

                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK

                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY

                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS

                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL

                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV

                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"

     polyA_signal    1781..1902

                     /note="SV40 poly(A) signal"

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(2321..2909)

                     /direction=LEFT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(3080..3940)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /note="AmpR"

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(3941..4045)

                     /gene="bla"

                     /note="AmpR promoter"

     rep_origin      4072..4527

                     /direction=RIGHT

                     /note="f1 ori"

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     polyA_signal    4658..4706

                     /note="synthetic polyadenylation signal"

     misc_feature    4720..4811

                     /note="pause site"

                     /note="RNA polymerase II transcriptional pause signal from 

                     the human?alpha-2 globin gene"

ORIGIN

        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg

       61 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg

      121 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag

      181 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc

      241 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg

      301 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg

      361 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa

      421 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag

      481 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc

      541 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat

      601 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca

      661 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct

      721 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc

      781 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact

      841 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag

      901 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta

      961 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa

     1021 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc

     1081 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt

     1141 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg

     1201 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt

     1261 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg

     1321 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac

     1381 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc

     1441 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt

     1501 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag

     1561 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag

     1621 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac

     1681 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa

     1741 ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt

     1801 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct

     1861 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt

     1921 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc

     1981 tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag ccttcaaccc

     2041 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt

     2101 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg

     2161 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa

     2221 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc

     2281 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc

     2341 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat

     2401 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc

     2461 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct

     2521 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg

     2581 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc

     2641 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga

     2701 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa

     2761 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta

     2821 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc

     2881 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg

     2941 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga

     3001 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg

     3061 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct

     3121 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg

     3181 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc

     3241 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa

     3301 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc

     3361 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt

     3421 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc

     3481 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt

     3541 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc

     3601 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt

     3661 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata

     3721 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga

     3781 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag

     3841 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa

     3901 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt

     3961 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga

     4021 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct

     4081 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg

     4141 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg

     4201 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac

     4261 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct

     4321 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt

     4381 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt

     4441 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt

     4501 ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc aactgttggg

     4561 aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa

     4621 gtaatattaa ggtacgggag gtacttggag cggccgcaat aaaatatctt tattttcatt

     4681 acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa

     4741 caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag

     4801 aacatttctc tatcgata