| 復(fù)制子 | pUC ori,f1 ori |
| 終止子 | SV40 poly(A) signal |
| 質(zhì)粒分類 | 哺乳細(xì)胞，信號(hào)通路報(bào)告載體 |
| 質(zhì)粒大小 | 4818bp |
| 原核抗性 | Amp |
| 克隆菌株 | DH5α |
| 培養(yǎng)條件 | 37℃，有氧 LB |
| 表達(dá)宿主 | 哺乳細(xì)胞 |
| 誘導(dǎo)方式 | 無須誘導(dǎo)，瞬時(shí)表達(dá) |
| 5'測(cè)序引物 | RVP3:CTAGCAAAATAGGCTGTCCC |
| 3'測(cè)序引物 | 根據(jù)序列設(shè)計(jì)引物 |
| 質(zhì)粒簡(jiǎn)介 | The pGL3 Luciferase Reporter Vectors(a–c) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, these Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation. |
| 質(zhì)粒序列 |
LOCUS Exported File 4818 bp ds-DNA circular SYN 23-3-2015 DEFINITION . ACCESSION . VERSION . KEYWORDS pGL3-basic SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4818) AUTHORS . TITLE Direct Submission JOURNAL Exported 2015-3-23 from MLCC FEATURES Location/Qualifiers source 1..4818 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 88..1740 /codon_start=1 /gene="luc+" /product="firefly luciferase" /EC_number=" " /note="luciferase" /note="enhanced?luc+ version of the luciferase gene" /protein_id=" " /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" polyA_signal 1781..1902 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(2321..2909) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3080..3940) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3941..4045) /gene="bla" /note="AmpR promoter" rep_origin 4072..4527 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" polyA_signal 4658..4706 /note="synthetic polyadenylation signal" misc_feature 4720..4811 /note="pause site" /note="RNA polymerase II transcriptional pause signal from the human?alpha-2 globin gene" ORIGIN 1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg 61 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg 121 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag 181 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc 241 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg 301 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg 361 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa 421 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag 481 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc 541 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat 601 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca 661 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct 721 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc 781 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact 841 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag 901 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta 961 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa 1021 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc 1081 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt 1141 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg 1201 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt 1261 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg 1321 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac 1381 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc 1441 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt 1501 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag 1561 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag 1621 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac 1681 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa 1741 ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt 1801 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct 1861 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt 1921 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc 1981 tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag ccttcaaccc 2041 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt 2101 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg 2161 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 2221 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 2281 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 2341 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 2401 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 2461 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct 2521 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 2581 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 2641 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 2701 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 2761 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 2821 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 2881 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 2941 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 3001 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 3061 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 3121 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 3181 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 3241 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 3301 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 3361 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 3421 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 3481 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 3541 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 3601 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 3661 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 3721 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 3781 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 3841 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 3901 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 3961 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 4021 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct 4081 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 4141 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 4201 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 4261 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 4321 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 4381 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 4441 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 4501 ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc aactgttggg 4561 aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa 4621 gtaatattaa ggtacgggag gtacttggag cggccgcaat aaaatatctt tattttcatt 4681 acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa 4741 caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag 4801 aacatttctc tatcgata |
