| 復(fù)制子 | p15A |
| 質(zhì)粒大小 | 3941bp |
| 原核抗性 | Amp,Kan |
| 克隆菌株 | DH5a |
| 培養(yǎng)條件 | 37度 |
| 5'測序引物 | 根據(jù)序列設(shè)計(jì) |
| 3'測序引物 | 根據(jù)序列設(shè)計(jì) |
| 備注 | 豐富的酶切位點(diǎn) |
| 質(zhì)粒宿主 | 大腸桿菌 |
| 質(zhì)粒用途 | 基因模板 |
| 片段類型 | ORF |
| 片段物種 | 空載體 |
| 原核抗性 | Amp,Kan |
| 質(zhì)粒簡介 | pACYC177 is an E. coli plasmid cloning vector containing the p15A origin of replication (1-4). This allows pACYC177 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell (5), but can be amplified with chloramphenicol or spectinomycin. Enzymes with unique restriction sites are shown in bold type and enzymes with two restriction sites are shown in regular type. Location of sites of all NEB restriction enzymes can be found on the NEB web site (choose Technical Reference > DNA Sequences and Maps). Restriction site coordinates refer to the position of the 5′-most base on the top strand in each recognition sequence. |
