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平臺編號：bio-109375
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質(zhì)粒信息： 宿主：DH5α，BL21
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規(guī)格：2ml甘油菌 20ul質(zhì)粒
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用途：病毒系列，腺病毒,兩個CMV啟動子，其中一個下游是多克隆位點，另外一個是熒光基因
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
pAdtrack-CMV
| 別稱 | Plasmid#16405 |
| 啟動子 | CMV |
| 復制子 | pUC |
| 終止子 | SV40 poly(A) signal |
| 質(zhì)粒大小 | 9237bp |
| 原核抗性 | Kan |
| 篩選標記 | EGFP |
| 克隆菌株 | Stbl3 |
| 培養(yǎng)條件 | 37度 |
| 誘導方式 | 無須誘導，瞬時表達 |
| 載體宿主 | 哺乳細胞,腺病毒 |
| 載體用途 | 蛋白表達 |
| 基因種屬 | 空載體 |
| 基因類型 | ORF |
| 原核抗性 | Kan |
| 熒光蛋白 | 綠色 |
| 質(zhì)粒簡介 | PAdTrack-CMV transgenic adenovirus vector to cell expression vector with CMV promoter and EGFP gene expression of green fluorescent protein tracking results, and use common pAdeasy1 vector maximum can be inserted into the 5.9kb, the use of common pAdeasy2 carrier can insert the 8.6kb. The specific use of the method can refer to the pAdeasy carrier system. Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We here report a strategy which simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, employing homologous recombination in bacteria rather than in eucaryotic cells. Following transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification.This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes. |
