載體基本信息
質(zhì)粒名稱:pMV306hsp+LuxG13
載體骨架基本信息
載體名稱:pMV306hsp
載體抗性:卡那霉素
空載體大小:4373 bp
完整質(zhì)粒大小:10559 bp
載體類型:細(xì)菌表達(dá)
高拷貝/低拷貝:低拷貝
生長條件:37 ℃
插入基因信息
插入基因名稱:Bacterial luciferase operon + G13 promoter
物種來源:Mycobacterium marinum + Photorhabdus luminescens
插入大小:6186 bp
基因又名:LuxABCDE
基因突變:It contains a gram-positive enhanced translation signal in front of luxA, luxC and luxE. G13 promoter cloned in front of luxC
克隆方法:限制性內(nèi)切酶
5' 克隆位點(diǎn):EcoRI(未破壞)
3' 克隆位點(diǎn):EcoRV(未破壞)
5' 測序引物agaataacgttggcactcgc
文獻(xiàn)引用方法
材料和方法部分:pMV306hsp+LuxG13 was a gift from Brian Robertson & Siouxsie Wiles (愛迪基因 plasmid # 26161)
參考文獻(xiàn)部分:Optimisation of bioluminescent reporters for use with mycobacteria. Andreu N, Zelmer A, Fletcher T, Elkington PT, Ward TH, Ripoll J, Parish T, Bancroft GJ, Schaible U, Robertson BD, Wiles S. PLoS One. 2010 May 24;5(5):e10777. 10.1371/journal.pone.0010777 PubMed 20520722
