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MITC-STO 拉丁名

(ATCC 56-X) (ATCC® 56-X.2™) 統(tǒng)一編號 

Organism: Mus musculus, mouse /

Cell Type: fibroblast /

Tissue: embryo /

Tissue embryo Cell Type fibroblast

Product Format 提供形式 frozen

Morphology fibroblast Culture Properties adherent

Biosafety Level 生物安全等級 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age embryo

Applications 用途 Mitomycin C-treated cells are used as a feeder layer

Storage Conditions 保藏條件 liquid nitrogen vapor phase

Comments 注釋 These cells are generated from mouse embryonic fibroblasts, STO cell line (ATCC CRL-1503), by a treatment of Mitomycin C.

These cells are provided to be used as feeder cells to support the growth of other cells. They have been treated with Mitomycin C and will not replicate.

The cells will begin to deteriorate in 2 to 3 weeks after plating. Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported.

Such populations are employed for maintenance of embryonal stem cells such as ES-D3 (ATCC CRL-1934) or teratocarcinoma stem cells (see ATCC CRL-1535 and ATCC CRL-1566) in the undifferentiated state.

It is recommended that the feeder cells be plated 24 hours before use at 6 x 106/T-75 or 2 x 106/T-25 in order to obtain a 100% confluent monolayer for stem cells growth.

Feeder layers are also employed to enhance the growth at low density populations of many hybridomas, colon carcinoma cell line (ATCC CCL-247) and squamous cell carcinoma cell lines (ATCC CRL-1624 and ATCC CRL-1629).

This action is due partly to conditioning of the substrate and the medium. A feeder layer confluence of 30 % is adequate and can be obtained by plating 2 x 106/T-75.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.

To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Cryopreservation Freeze Medium:

Complete growth medium supplemented with 5% (v/v) DMSO

Storage Temperature 保藏溫度 : Liquid nitrogen vapor phase

Culture Conditions 培養(yǎng)條件

Temperature 培養(yǎng)溫度 : 37℃

Atmosphere 需氧情況 : Air, 95%; carbon dioxide (CO2), 5%

Name of Depositor 寄存人 ATCC

References  參考文獻 Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416

Teratomas and Differentiation, eds. Michael I. Sherman and David Solter, 13–14. New York: Academic Press, 1975.

Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624