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名稱 HS-27A (人骨髓細胞) (STR鑒定正確) 

別稱 HS27A; HS27a 

種屬 人類 

生長特性 貼壁細胞 

細胞形態(tài) 成纖維細胞樣 

凍存條件 凍存液：55% 基礎培養(yǎng)基+40% FBS+5% DMSO溫度：液氮 

培養(yǎng)方案A（默認） 生長培養(yǎng)基:RPMI-1640+10%FBS+1%P/S培養(yǎng)條件:氣相：空氣，95%；CO2，5%, 溫度：37℃ 

推薦傳代比例 1:2-1:3 

推薦換液頻率 2-3次/周 

參考資料(來源文獻)

背景描述 HS-27A is a fibroblast cell line that was isolated in (year from History) from the stroma of a 30-year-old White male donor. HS-27A is a subclone of HS-27.HS-27A forms large flattened polygonal shaped cells that exemplify "blanket" cells and maintain numerous intercellular contacts with neighboring cells. Unlike HS-5, HS-27A secretes low levels of growth factors and does not support proliferation of isolated progenitor cells in cocultures. HS-27A expresses relatively high levels of vascular cell adhesion molecule 1 (VCAM-1). 

年齡（性別） 男性；30歲 

組織來源 骨頭；骨髓；基質 

細胞類型 轉化細胞系 

生物安全等級 BSL-2 

倍增時間 ~36h-48 hours 

抗原表達情況 Vascular cell adhesion molecule 1 (CD106, VCAM-1) 

應用領域 3D cell culture； Immunology 

保藏機構 ATCC; CRL-2496